Un’alternativa all’uso del PVP (poli vinil pirrolidone) per la selezione degli spermatozoi da utilizzare al momento della ICSI è quella dell’acido ialuronico. L’uso del PVP permette di rallentare i movimenti dello spermatozo e di osservare lo spermatozoo morfologicamente migliore per il suo utilizzo nella microiniezione tenendo presente, altresì che tale sostanza potrebbe avere effetti tossici sull’ovocita.
Se si usa l’acido ialuronico è possibile effettuare una migliore selezione, in quanto nella fecondazione naturale l’acido Ialuronico è presente sulla superfice degli ovociti e, gli spermatozoi al momento della fecondazione devono possedere dei recettori per l’acido ialuronico affinchè vi sia il legame con l’ovocita e quindi la penetrazione nel suo interno. Affinchè avvenga questo colloquio recettoriale è necessario che lo spermatozoo abbia caratteri di normalità del suo DNA e dei cromosomi: il DNA deve essere integro e senza frammentazioni. Nel caso di PMA : al momento della ICSI, si crea un contatto tra gli spermatozoi da microiniettare negli ovociti e l’acido ialuronico che seleziona in un modo facilmente osservabile gli spermatozoi con DNA non danneggiato che possono quindi essere bloccati e iniettati singolarmente in un ovocita maturo (MII). L’uso dell’acido ialuronico, quindi permette di effettuare una ICSI che non utilizza il PVP ed evita i sui possibili effetti tossici sull’ovocita e nello stesso tempo permette una selezione di spermatozoi con DNA non frammentato. Nell’ambito del MASTER di II livello di Biotecnologie della Riproduzione che si tiene presso il dipartimento di Scienze per la Vita della II Università di Napoli, sede di Caserta si sta sperimentando tale tecnica usando modelli animali in un ambito collaborativo dove sono coinvolte strutture della facoltà che studiano la frammentazione del DNA degli spermatozoi. Un work in progress per dar modo agli studenti del Master di II livello di apprendere tale nuova tecnologia.
Hey! This is kind of off topic but I need some advice from an established blog.
Is it difficult to set up your own blog? I’m not very techincal but I can figure things out pretty
fast. I’m thinking about setting up my own but I’m not sure
where to start. Do you have any points or suggestions?
Vous pouvez vous connecter through Facebook ou créer un compte utilisateur,
selon votre choix.
Ahaa, its fastidious discussion on the topic of this post here at this webpage, I have read all that, so at this time me also commenting at this place.
Thanks a lot for sharing this with all folks you really know what you’re talking approximately!
Bookmarked. Kindly also consult with my site =). We may have a link exchange agreement
It’s going to be end of mine day, except before finish I am reading this wonderful paragraph to
improve my knowledge.
Nice post. I was checking constantly this blog and
I’m impressed! Very helpful information particularly the last part 🙂 I care for such info a
lot. I was looking for this particular info for a long time.
Thank you and best of luck.
We’re a group of volunteers and starting a new scheme in our community.
Your web site provided us with valuable info to work on.
You have done an impressive job and our whole community will be thankful to you.
I’m really enjoying the design and layout of your site.
It’s a very easy on the eyes which makes it much more enjoyable
for me to come here and visit more often. Did you hire out a developer to create
your theme? Excellent work!
I am curious to find out what blog system you’re using?
I’m having some small security problems with my latest blog and I would like to find something more
risk-free. Do you have any solutions?
I couldn’t resist commenting. Perfectly written!
each time i used to read smaller articles or reviews which also
clear their motive, and that is also happening with
this piece of writing which I am reading here.
Superb, what a blog it is! This web site provides helpful information to us,
keep it up.
I am extremely impressed with your writing skills and also with the
layout on your blog. Is this a paid theme or did you customize it yourself?
Anyway keep up the excellent quality writing, it’s rare to see a nice
blog like this one today.
Hey There. I found your blog using msn. This is
an extremely well written article. I will make sure to bookmark it and come back to read more
of your useful information. Thanks for the post. I’ll definitely comeback.
Hola muchas felicitaciones por la información! Era justo lo que estaba buscando, sigue así me encanta tu
magnificent publish, very informative. I wonder why the opposite experts of this sector don’t realize this.
You should proceed your writing. I’m sure, you have a great readers’ base already!
Should you’ve acquired any problems with app
just write us. You too can use Empire Z hack online,
learn under and click on generate for those who favor that manner.
Whats up are using WordPress for your blog platform?
I’m new to the blog world but I’m trying to get started and create my own. Do you require
any html coding knowledge to make your own blog?
Any help would be really appreciated!
Greetings from Ohio! I’m bored at work so I decided to check
out your website on my iphone during lunch break.
I really like the knowledge you present
here and can’t wait to take a look when I get home. I’m surprised at how quick your blog loaded on my phone ..
I’m not even using WIFI, just 3G .. Anyways, good site!
It’s a pity you don’t have a donate button! I’d definitely donate to
this brilliant blog! I suppose for now i’ll settle for book-marking and adding your RSS feed to my Google account.
I look forward to new updates and will share this website with my Facebook group.
Thanks for another informative web site. The place else could
I get that kind of information written in such an ideal approach?
I’ve a mission that I’m simply now working on, and I’ve been on the glance out for such info.
I’m not that much of a online reader to be honest but your sites really nice, keep it up!
I’ll go ahead and bookmark your site to come back down the road.
I was suggested this web site by means of my cousin. I am no longer sure whether or not this publish is written by means of
him as nobody else recognise such distinct approximately my trouble.
You are incredible! Thank you!
I think this is among the most vital information for me.
And i’m glad reading your article. But want to remark on few general things,
The web site style is ideal, the articles is really nice : D.
Good job, cheers
My spouse and I absolutely love your blog and find the majority of your post’s to be just what I’m looking for.
can you offer guest writers to write content for yourself?
I wouldn’t mind publishing a post or elaborating on some
of the subjects you write related to here. Again, awesome blog!
Superb post however I was wanting to know if you could write a litte more on this
topic? I’d be very grateful if you could elaborate a little bit more.
I think this is among the such a lot vital information for me.
And i’m happy studying your article. However wanna statement on few common things, The website taste is great,
the articles is really nice : D. Excellent task, cheers
Nice response in return of this issue with solid arguments and telling everything
on the topic of that.
This validity is obtained after checking them inside a database so the customers of this generator can rest assured that these
are genuine free Microsoft Components , ready for
use each time you might be prepared.
Magnificent site. Plenty of useful info here.
I am sending it to some pals ans additionally sharing in delicious.
And obviously, thank you on your effort!
Excellent beat ! I would like to apprentice while you amend
your site, how could i subscribe for a weblog site? The account helped me
a acceptable deal. I had been a little bit acquainted of this your broadcast offered shiny transparent idea
This info is invaluable. When can I find out
When I initially commented I clicked the “Notify me when new comments are added” checkbox and now each time a comment is added I get several emails with
the same comment. Is there any way you can remove me from that service?
Thanks a lot!
This trend has been observed in the past, and will
set new standards in the malware industry. A lot of stories happen when Lilo’s family has a new member, Stitch.
s a breakaway success, with little sign of slowing.
Mais nous-mêmes sommes heureux de vous-même dire o qual notre objet de tromperie
pour clash of clans est désormais mis à jour!
En nuestra tienda encontrará la pieza ideal que servirá para prosperar su figura para cada temporada.
Hi, its fastidious paragraph regarding media print, we all
understand media is a enormous source of information.
Your way of describing everything in this paragraph is in fact good,
all be able to simply know it, Thanks a lot.
I simply could not depart your site prior to suggesting that I extremely loved
the standard info an individual provide on your guests?
Is gonna be back frequently to inspect new posts
Fastidious respond in return of this query with solid arguments and explaining the whole thing on the topic of that.
Yes! Finally something about dog tshirts.
At this time I am going away to do my breakfast, later than having my breakfast coming over again to read additional news.
Somebody necessarily help to make critically articles I’d state.
That is the very first time I frequented your web page and thus far?
I amazed with the analysis you made to make this actual put up extraordinary.
It’s hard to come by educated people about this subject, but you sound like you know what you’re talking about!
Hi are using WordPress for your site platform? I’m new to the blog world
but I’m trying to get started and set up my own. Do you require any html coding expertise to make your own blog?
Any help would be really appreciated!
I know this site provides quality dependent content
and extra information, is there any other web site
which offers these stuff in quality?
En fait, le vin est tres sensible aux variations de ses conditions de conservation.
An interesting discussion is worth comment. I believe that you ought to publish
more on this issue, it may not be a taboo subject but usually people don’t talk about these subjects.
To the next! Kind regards!!
This hack features a complete lot of options,
some of which really annoy different users in the event you use them maliciously.
Hurrah, that’s what I was seeking for, what a
information! present here at this weblog, thanks admin of this site.
Helpful info. Fortunate me I discovered your site by accident, and I am stunned
why this coincidence did not took place in advance! I bookmarked it.
Somebody essentially lend a hand to make critically articles I might
state. That is the first time I frequented your web
page and thus far? I amazed with the research you made to create this actual post incredible.
Wow, this paragraph is good, my younger sister is analyzing these kinds of things, thus I am going to let know
Hey there just wanted to give you a quick heads up.
The words in your post seem to be running off
the screen in Ie. I’m not sure if this is a format issue or something to do with web browser compatibility but I figured
I’d post to let you know. The design and style look
great though! Hope you get the problem solved soon.
Nice blog here! Also your web site loads up very fast!
What web host are you using? Can I get your affiliate link to your
host? I wish my site loaded up as fast as yours lol
Terrific work! That is the kind of information that are supposed to be
shared across the web. Shame on Google for no longer positioning this put up upper!
Come on over and consult with my web site . Thanks =)
I have been browsing on-line greater than three hours these days,
yet I never found any fascinating article like yours.
It is lovely price enough for me. Personally, if all site owners
and bloggers made good content material as you probably did, the net might be a lot more useful than ever before.
Everything is very open with a very clear explanation of the issues.
It was really informative. Your website is useful.
Thank you for sharing!
My brother recommended I may like this web site.
He was totally right. This put up actually made my day.
You cann’t consider just how a lot time I had spent for this
information! Thank you!
I like the helpful information you provide in your articles.
I will bookmark your weblog and check again here frequently.
I am quite sure I will learn many new stuff right here! Good luck for the next!
Thank you for sharing your thoughts. I truly
appreciate your efforts and I will be waiting for your next post thanks once again.
The King Post is a board that runs directly vertical from the
top of the bottom chord to the underside of the top chords
where they meet in the center of the shed. You also need to consider security for
your metal garage. Fireworks and pyrotechnics lit up the stage during One, easily one of Metallica’s finest songs, and the video screens showed
the picture in black and white, likely as a nod to the song’s monochrome music video.
Hello to every one, the contents existing at this
site are genuinely amazing for people experience,
well, keep up the good work fellows.
Au deballage, ma reaction etait : voici un guide de percage tres bien pense et fait pour durer.
Hi there all, here every one is sharing such experience, therefore it’s nice to read this web site, and I used to pay a visit this webpage every day.
Hello there! This is my first visit to your
blog! We are a team of volunteers and starting a new initiative in a community
in the same niche. Your blog provided us beneficial information to work
on. You have done a outstanding job!
Ce guide n’a pas pour but de vous donner des adresses d’hebergements
ou autres details pratiques.
Just desire to say your article is as astonishing. The clarity in your post
is just spectacular and i can assume you are an expert on this subject.
Well with your permission let me to grab your feed to keep updated
with forthcoming post. Thanks a million and please
carry on the enjoyable work.
Hey there! Someone in my Facebook group shared this site with us so I came to take a look.
I’m definitely enjoying the information. I’m bookmarking and will
be tweeting this to my followers! Excellent blog and superb design.
Do you mind if I quote a couple of your posts
as long as I provide credit and sources back to your website?
My blog site is in the exact same niche as yours and my visitors
would definitely benefit from some of the information you present here.
Please let me know if this alright with you.
I was very happy to find this website. I want to to thank you for your time for this fantastic read!!
I definitely really liked every part of it and i also have you book marked to look at new information on your blog.
The hackers have made available the accomplished software which users just download, do the set up on their products, and commence managing
Fascinating blog! Is your theme custom made or did you download it from somewhere?
A theme like yours with a few simple tweeks would really make
my blog stand out. Please let me know where you got your design. Kudos
Howdy! I know this is kinda off topic but I’d figured I’d
ask. Would you be interested in trading links or maybe guest writing a blog article
or vice-versa? My blog discusses a lot of the same topics as yours and
I feel we could greatly benefit from each other.
If you are interested feel free to shoot me an email.
I look forward to hearing from you! Awesome blog by the
Thanks very interesting blog!
Thanks for finally talking about > MASTER II livello – Biotecnologie Applicate alla Riproduzione
Assistita : ICSI e l
I don’t even understand how I ended up right here, but I thought this publish was great.
I don’t know who you are but certainly you are going to a well-known blogger in the
event you aren’t already. Cheers!
Excellent web site you have got here.. It’s hard to find
high-quality writing like yours these days.
I honestly appreciate individuals like you! Take care!!
Aw, this was an incredibly good post. Taking a few minutes and actual effort to create
a great article… but what can I say… I procrastinate a lot and don’t
seem to get anything done.
Nice blog! Is your theme custom made or did you download it from
somewhere? A theme like yours with a few simple tweeks
would really make my blog jump out. Please let me know
where you got your theme. Thank you
I think the admin of this web page is really working hard for his web site, since here every information is quality based information.
From this article aims at providing you with their grosfillex bahia chaise lounge current interior design, you are going to need to be.
I am really enjoying the theme/design of your site.
Do you ever run into any browser compatibility issues? A couple of my blog readers have complained about my site not operating correctly in Explorer but looks great in Safari.
Do you have any advice to help fix this problem?
I all the time used to read article in news papers but now as I am a user
of internet so from now I am using net for content, thanks to web.
certainly like your web-site however you need to test the spelling on quite a few
of your posts. A number of them are rife with spelling problems and I in finding it very troublesome to tell the reality nevertheless I’ll surely come again again.
This paragraph will help the internet people for creating new web site or
even a blog from start to end.
I think this is among the most significant info for me.
And i’m glad reading your article. But want to remark on few general
things, The site style is ideal, the articles is really excellent : D.
Good job, cheers
Great weblog here! Also your website lots up fast!
What web host are you the use of? Can I get
your affiliate hyperlink for your host? I want my web site loaded up as
quickly as yours lol
I think the admin of this web page is genuinely working hard for his web page, for the reason that here every information is quality based data.
Every weekend i used to go to see this web site, because i
want enjoyment, since this this web page conations really
fastidious funny information too.
It’s perfect time to make some plans for the longer term and
it is time to be happy. I have learn this submit and if I may
I desire to counsel you few interesting issues or advice. Perhaps
you can write subsequent articles relating to this article.
I desire to learn even more things approximately it!
I like the helpful information you provide in your
articles. I will bookmark your weblog and check again here
regularly. I’m quite sure I will learn many new stuff right here!
Good luck for the next!
Hello, I enjoy reading through your post. I like to write
a little comment to support you.
Thank you for sharing your thoughts. I really appreciate your efforts and I
am waiting for your next write ups thank you once again.
Hey there, You’ve done a great job. I will definitely digg it and personally
recommend to my friends. I’m sure they will be benefited from
this web site.
Hi, just wanted to tell you, I liked this post.
It was inspiring. Keep on posting!
Helpful info. Lucky me I discovered your web site
accidentally, and I’m shocked why this coincidence didn’t took place in advance!
I bookmarked it.
This blog was… how do you say it? Relevant!! Finally I’ve found something which helped me.
Hey! I know this is somewhat off topic but I was wondering which blog platform are you using for this site?
I’m getting fed up of WordPress because I’ve hhad problems with hackers and I’m looking at alternatives
for another platform. I would be great if you could point me in the direction of
a good platform.
Maintain your list restricted to anybody who you would want to
see in the pictures you will be searching at for
the rest of your life.
Neat blog! Is your theme custom made or did you download it from somewhere?
A theme like yours with a few simple adjustements would really make my blog shine.
Please let me know where you got your design. Thank you
We stumbled over here from a different web address and thought
I might check things out. I like what I see so now i am following you.
Look forward to looking at your web page again.
Thank you for sharing your thoughts. I really appreciate your efforts and I am waiting for your further write
ups thank you once again.
Information to Shopping for Shares: This section is
a comprehensive guide to easy methods to get started with shopping for stocks on-line.
Tengo dos preguntas, la más importante: Compraré arcilla verde para darmela en mascarilla en tanto que he oido que es muy buena y tengo curiosidad.
Attractive section of content. I simply stumbled upon your site and in accession capital
to claim that I get actually enjoyed account your weblog posts.
Anyway I will be subscribing in your feeds
or even I fulfillment you get admission to constantly quickly.
What’s up to all, how is the whole thing, I think every one is getting more
from this site, and your views are fastidious in support of new users.
Hello, I desire to subscribe for this webpage to obtain latest updates,
thus where can i do it please help out.
I do not even know how I ended up here, but I
thought this post was great. I don’t know who you are but certainly you’re going to a famous blogger if you are not already ;
Great product, and cheaper than the opponents!
I do trust all the ideas you have introduced on your post.
They are really convincing and can certainly work. Still, the posts are too short
for newbies. May just you please prolong them a little from
subsequent time? Thanks for the post.
I am extremely impressed with your writing abilities as smartly as with the structure to your weblog.
Is this a paid topic or did you modify it your self?
Anyway stay up the excellent high quality writing, it is rare to peer a great weblog like this one nowadays..
On no less than an annual basis the Financial institution will provide social media coaching to
Pretty part of content. I simply stumbled upon your web site and
in accession capital to say that I get actually enjoyed account your blog posts.
Any way I will be subscribing on your augment and even I fulfillment you get right
of entry to constantly quickly.
No matter if some one searches for his vital thing, thus he/she
wishes to be available that in detail, so that thing is maintained over here.
I am genuinely thankful to the holder of this web page who has shared this impressive piece
of writing at here.
I know this web site presents quality based posts and additional information, is there any other web site which presents such information in quality?
When someone writes an post he/she maintains the image of a user in his/her mind
that how a user can know it. Therefore that’s why this article is great.
Thanks for sҺaring ƴour thoughts about anatomia.
Pretty! This was a really wonderful post. Many thanks for supplying this info.
It’s difficult to find experienced people about this subject,
however, you sound like you know what you’re talking about!
Very great post. I simply stumbled upon your blog and wished to mention that I have
really enjoyed browsing your blog posts. After all I’ll be subscribing in your feed and I’m hoping you write
again very soon!
I blog quite often and I genuinely appreciate your content.
Your article has truly peaked my interest.
I’m going to take a note of your website and keep checking
for new details about once per week. I opted in for your Feed as well.
I enjoy, result in I found just what I was looking for.
You’ve ended my 4 day long hunt! God Bless you man. Have a nice day.
I really like what you guys are up too. This sort of
clever work and exposure! Keep up the superb works guys I’ve incorporated
you guys to our blogroll.
whoah this blog is magnificent i really like studying your articles.
Stay up the good work! You know, many persons are searching
round for this info, you could aid them greatly.
Excellent site. Plenty of helpful information here. I’m sending it to several buddies ans also sharing in delicious.
And obviously, thank you on your effort!
After I initially left a comment I appear to have clicked
on the -Notify me when new comments are added- checkbox and now
every time a comment is added I recieve 4 emails with the exact same comment.
There has to be a means you are able to remove me from that service?
Thanks a lot for sharing this with all of us you actually recognize what you’re talking approximately!
Bookmarked. Please also visit my site =). We can have a hyperlink change contract among
What’s up, I wish for to subscribe for this weblog to obtain newest updates, therefore where can i do it please help out.
Yes! Finally something about Angst und Schrecken in Las Vegas.
Free Expansion Seashore Hack innovative cheat developed by simply for all lovers Progress Seashore.
Fantastic beat ! I wish to apprentice while you amend your
website, how can i subscribe for a blog website? The
account helped me a acceptable deal. I had been tiny bit familiar of this your broadcast provided vivid transparent idea
Hey are using WordPress for your site platform? I’m new to the blog world
but I’m trying to get started and create my own. Do you require any coding
expertise to make your own blog? Any help
would be greatly appreciated!
Great weblog here! Additionally your web site so much up very
fast! What web host are you using? Caan I am getting your associate
hyperlink to your host? I wish my web site loaded up as quickly as yours lol
Good day! This is my first visit to your blog!
We are a collection of volunteers and starting a new project
in a community in the same niche. Your blog provided us valuable information to work on. You
have done a marvellous job!
It’s really a cool and useful piece of info. I’m satisfied
that you just shared this helpful information with
us. Please keep us informed like this. Thank you for
I’m gone to inform my little brother, that he should also visit this weblog on regular basis to get updated from most recent reports.
I am regular visitor, how are you everybody? This piece of writing posted at this site is in fact good.
I visited several websites but the audio feature for audio songs present
at this website is genuinely excellent.
Great post! We will be linking to this great post
on our site. Keep up the good writing.
Thanks for finally writing about > MASTER II livello – Biotecnologie Applicate
alla Riproduzione Assistita : ICSI e l
I feel that is one of the such a lot significant information for me.
And i’m satisfied studying your article. But should statement
on few normal things, The web site taste is great, the articles is in point of fact great : D.
Excellent activity, cheers
This text is priceless. When can I find out more?
Thanks on your marvelous posting! I really enjoyed reading
it, you could be a great author.I will be sure to bookmark your blog and will often come back from
now on. I want to encourage that you continue
your great writing, have a nice evening!
Wow, awesome blog format! How lengthy have you ever been blogging for?
you made running a blog glance easy. The total glance of your web site
is magnificent, let alone the content!
Hi just wanted to give you a quick heads up
and let you know a few of the pictures aren’t loading correctly.
I’m not sure why but I think its a linking issue. I’ve tried it
in two different browsers and both show the same outcome.
Thank you for the good writeup. It in reality was a leisure account it.
Look advanced to far added agreeable from you! However, how could we keep up
When someone writes an paragraph he/she maintains the image of a user
in his/her brain that how a user can be aware of it.
Therefore that’s why this piece of writing is great. Thanks!
Hi there! This post couldn’t be written much better!
Going through this post reminds me of my previous roommate!
He constantly kept talking about this. I will send this information to him.
Fairly certain he’s going to have a very good read. Many thanks for sharing!
Undeniably believe that which you stated. Your favorite reason appeared to be on the internet
the easiest thing to be aware of. I say to
you, I definitely get annoyed while people think about worries that
they just don’t know about. You managed to hit the nail
upon the top and also defined out the whole thing without having side-effects , people can take a signal.
Will likely be back to get more. Thanks
Do you mind if I quote a couple of your articles as long as I provide credit and
sources back to your webpage? My website is in the
exact same niche as yours and my users would genuinely benefit
from some of the information you present here.
Please let me know if this ok with you. Thanks!
Great blog! Is your theme custom made or
did you download it from somewhere? A design like yours with a few simple tweeks would really make my blog shine.
Please let me know where you got your design. Appreciate it
Your mode of telling everything in this article is truly nice, all be capable of effortlessly understand it, Thanks a lot.
Good blog you have here.. It’s difficult to find high-quality writing like yours these
days. I seriously appreciate individuals like you!
Awesome things here. I’m very glad to look your post.
Thank you so much and I’m taking a look ahead to contact you.
Will you kindly drop me a mail?
I visited many web sites except the audio quality for audio songs present at this site is actually marvelous.
Hi would you mind letting me know which web host you’re using?
I’ve loaded your blog in 3 different browsers and I must say this blog loads a lot faster then most.
Can you recommend a good internet hosting provider at a
reasonable price? Thanks a lot, I appreciate it!
Post writing is also a fun, if you know then you can write if not it is complex to write.
When someone writes an article he/she retains the plan of a user in his/her brain that how
a user can understand it. Therefore that’s why this piece of writing is perfect.
Good day! I just would like to give you a huge
thumbs up for the excellent info you have
got right here on this post. I’ll be returning to your web site for more soon.
Hello very cool website!! Man .. Beautiful ..
Amazing .. I’ll bookmark your site and take the feeds additionally?
I’m happy to seek out numerous useful information right here within the put up, we’d like
develop more strategies on this regard, thanks for sharing.
. . . . .
Hi there, this weekend is pleasant in support of
me, as this occasion i am reading this great educational paragraph here at
Greetings! Very useful advice in this particular post!
It’s the little changes which will make the biggest changes.
Thanks for sharing!
Hey very interesting blog!
Does your blog have a contact page? I’m having problems locating
it but, I’d like to send you an email. I’ve got some recommendations for your blog you might be
interested in hearing. Either way, great website and I look forward to seeing it improve over time.