Andrologia

Sperm mucus penetration test

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TEST DI PENETRAZIONE DEGLI SPERMATOZOI : Sperm Penetration Tests

TESTO DI : FULVIO CESARONI seminologo /embriologo

Il test di penetrazione degli spermatozoi in vitro, permette di valutare se gli spermatozoi di un paziente sono capaci di penetrare il muco cervicale e raggiungere le salpingi: dove nel terzo medio incontrano l’ovocita maturo in fase M2 e fecondarlo.

  • il Test di penetrazione  nel muco cervicale   saggia la capacità degli spermatozoi a penetrare e conservare la propria motilità
  • Se si nota, in vitro, un movimento shaking degli spermatozoi si ipotizza la presenza di auto-anticorpi (ASA) diretti verso gli spermatozoi . Il test deve essere completato dal MAR test e dall’IBT.
  • quando il PCT post coital test ha dimostrato che gli spermatozoi si fermano a contatto col muco cervicale o mostrano fenomeni di shaking.

 

Sperm mucus penetration test TEST DI PENETRAZIONE DEGLI SPERMATOZOI NEL MUCO CERVICALE

la donna deve essere in periodo ovulatorio

controllo dell’LH

FERN test (felcizzazione del muco cervicale = dimostra una maturità del muco in periodo ovulatorio, rendendo il muco penetrabile agli spermatozoi e rendendo possibile la loro capacitazione in vivo)

Muco cervicale: raccolta del muco cervicale in periodo periovulatorio (FERN test positivo) tramite una cannula sottile (aspiglaire).

Uomo : raccolta del liquido seminale dopo il prelievo del muco cervicale

è necessario un campione seminale dopo  2-5 gg  di astinenza

In laboratorio:

TEST DIRETTO:  al muco cervicale strisciato sottilmente su un vetrino si aggiunge qualche microlitro di liquido seminale ben omogeneizzato.  Dopo circa 90 minuti si osserva se il muco cervicale è riuscito a penetrare il muco cervicale e a quale distanza.Inoltre si osserva se ci sono fenomeni di shaking.

TEST CROCIATO: può essere fatto anche un test crociato usando muco cervicale di donatrice e spermatozoi del paziente, oppure muco cervicale della partner e liquido seminale da donatore. Tale test permette di vedere se fenomeni di shaking sono dovuti ad autoanticorpi del paziente o della partner.

Sperm mucus penetration test

si possono avere i seguenti risultati

TEST DIRETTO : Normalmente gli spermatozoi penetrano il muco cervicale e si muovono in esso con motilità progressiva.

TEST DIRETTO ANORMALE : Gli spermatozoi non riescono a penetrare il muco cervicale o se lo penetrano si arrestano o si muovono con movimento sheking, o talvolta formano agglutinati specialmente testa-testa. Ciò dimostra la presenza di anticorpi antispermatozoo, che possono essere presenti sia nel muco cervicale o sugli spermatozoi. Una indagine accurata sul liquido seminale può dimostrare la presenza di anticorpi antispermatozoo. (MAR TEST, IBT TEST)

RISULTATI

TEST CROCIATO:

-gli spermatozoi di donatore penetrano nel muco cervicale ma non quelli del partner maschile (test anomalo spermatozoi del partner maschile = paziente)

-gli spermatozoi del partner non penetrano nel muco cervicale della donatrice. (test anomalo spermatozoi del paziente)

-gli spermatozoi del partner e del donatore non riescono a penetrare nl muco cervicale della partner.(test anomalo per il muco della paziente)

 

 


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