Home Andrologia Sperm mucus penetration test

Sperm mucus penetration test

Da dottvolpicelli

Ultimo aggiornamento  2021-11-16 15:49:26

TEST DI PENETRAZIONE DEGLI SPERMATOZOI: Sperm Penetration Test

Il test di penetrazione spermatozoo-muco in vitro (SMPT) è un test di funzionalità dello sperma che misura la capacità dello sperma di penetrare il muco cervicale e raggiungere le salpingi: dove nel terzo medio incontrano l’ovocita maturo in fase M2 e fecondarlo.

  • il Test di penetrazione  nel muco cervicale   saggia la capacità degli spermatozoi a penetrare e conservare la propria motilità
  • Se si nota, in vitro, un movimento shaking degli spermatozoi si ipotizza la presenza di auto-anticorpi (ASA) diretti verso gli spermatozoi . Il test deve essere completato dal MAR test e dall’IBT.
  • quando il PCT post coital test ha dimostrato che gli spermatozoi si fermano a contatto col muco cervicale o mostrano fenomeni di shaking.

la donna deve essere in periodo ovulatorio

controllo dell’LH

FERN test (felcizzazione del muco cervicale = dimostra una maturità del muco in periodo ovulatorio, rendendo il muco penetrabile agli spermatozoi e rendendo possibile la loro capacitazione in vivo)

Muco cervicale: raccolta del muco cervicale in periodo periovulatorio (FERN test positivo) tramite una cannula sottile (aspiglaire).

Uomo: raccolta del liquido seminale dopo il prelievo del muco cervicale

è necessario un campione seminale dopo  2-5 gg  di astinenza

In laboratorio:

TEST DIRETTO:  al muco cervicale strisciato sottilmente su un vetrino si aggiunge qualche microlitro di liquido seminale ben omogeneizzato.  Dopo circa 90 minuti si osserva se lo sperma è riuscito a penetrare nel muco cervicale e a quale distanza. Inoltre si osserva se ci sono fenomeni di “shaking” (gli spermatozoi sono incapaci di progredire, mantengono ferma la testa mentre la coda si muove freneticamente).

Noi effettuiamo il test utilizzando un capillare rettangolare millimetrato la cui estremità inferiore è immersa in una provetta in cui sono posti 50 μl di seme ed altrettanti di medium di coltura (v. disegno sottostante).

TEST CROCIATO: può essere fatto anche un test crociato usando muco cervicale di donatrice e spermatozoi del paziente, oppure muco cervicale della partner e liquido seminale da donatore. Tale test permette di vedere se fenomeni di shaking sono dovuti ad autoanticorpi del paziente o della partner.

RISULTATI 

TEST DIRETTO: Normalmente gli spermatozoi penetrano il muco cervicale e si muovono in esso con motilità progressiva.

TEST DIRETTO ANORMALE: Gli spermatozoi non riescono a penetrare il muco cervicale o se lo penetrano si arrestano o si muovono con movimento shaking, o talvolta formano agglutinati specialmente testa-testa. Ciò dimostra la presenza di anticorpi antispermatozoo, che possono essere presenti nel muco cervicale o sugli spermatozoi. Una indagine accurata sul liquido seminale può dimostrare la presenza di anticorpi antispermatozoo. (MAR TEST, IBT TEST)

TEST CROCIATO:

-gli spermatozoi di donatore penetrano nel muco cervicale ma non quelli del partner maschile (patologia spermatica del partner maschile)

-gli spermatozoi del partner e del donatore non riescono a penetrare nl muco cervicale della partner.(patologia del muco cervicale della paziente).

 

 

Potrebbe piacerti anche

37 commenti

my latest blog post 22 Giugno 2015 - 21:02

I have been browsing on-line more than three hours these days,
yet I by no means discovered any fascinating article like yours.

It’s beautiful price enough for me. Personally, if all website owners and bloggers made excellent content as you
did, the net will likely be a lot more helpful than ever before.

Rispondi
why not try this out 23 Giugno 2015 - 4:12

Hey There. I discovered your blog using msn. That is a really smartly written article.
I’ll be sure to bookmark it and come back to read more of your useful
information. Thanks for the post. I’ll definitely comeback.

Rispondi
tree service virginia beach 23 Giugno 2015 - 6:39

It is truly a great and helpful piece of information. I am glad that you
just shared this helpful information with us. Please stay us informed like this.
Thank you for sharing.

Rispondi
underwater welding academy 30 Giugno 2015 - 4:54

I’m really enjoying the design and layout of your blog. It’s a very easy
on the eyes which makes it much more enjoyable for me to come
here and visit more often. Did you hire out a developer to create your
theme? Outstanding work!

Rispondi
average salary of an underwater welder 30 Giugno 2015 - 5:13

Thank you for the auspicious writeup. It in fact was
a amusement account it. Look advanced to more added agreeable from you!

By the way, how could we communicate?

Rispondi
underwater welding jobs in california 30 Giugno 2015 - 7:39

Terrific article! That is the type of information that are
supposed to be shared around the net. Shame on the seek engines for now not positioning this publish higher!
Come on over and seek advice from my site . Thanks =)

Rispondi
future of welding 30 Giugno 2015 - 9:38

We absolutely love your blog and find many of your post’s
to be just what I’m looking for. Would you offer guest writers to write
content for you personally? I wouldn’t mind publishing a post or elaborating on some of the subjects you write regarding here.
Again, awesome blog!

Rispondi
welding occupations 30 Giugno 2015 - 10:42

My spouse and I stumbled over here by a different web address and thought I might check things out.
I like what I see so now i’m following you.
Look forward to finding out about your web page again.

Rispondi
average salary welder 30 Giugno 2015 - 18:03

It’s a shame you don’t have a donate button! I’d most certainly donate to this
excellent blog! I guess for now i’ll settle for bookmarking and
adding your RSS feed to my Google account. I look forward to brand new
updates and will share this website with my Facebook group.

Talk soon!

Rispondi
hbot treatment 30 Giugno 2015 - 20:59

Do you have a spam issue on this blog; I also am a blogger, and I was wondering
your situation; many of us have created some nice practices
and we are looking to exchange techniques with others, why not shoot me an email
if interested.

Rispondi
the welding encyclopedia 30 Giugno 2015 - 22:48

Hi, this weekend is nice in support of me, because this occasion i am reading this fantastic educational paragraph here at my house.

Rispondi
https://docs.google.com/ 2 Luglio 2015 - 14:15

There are many different kinds of games there is to find right boom
beach offline one for your mobile that will definitely create
a relaxing past time.

Rispondi
single family residence defined 10 Luglio 2015 - 18:10

This website was… how do you say it? Relevant!!
Finally I have found something which helped me. Cheers!

Rispondi
click the link 10 Luglio 2015 - 20:37

Actually when someone doesn’t be aware of after that its up
to other people that they will assist, so here it
happens.

Rispondi
market research 10 Luglio 2015 - 23:47

Its such as you read my thoughts! You seem
to know a lot approximately this, like you wrote the e-book in it or something.
I think that you simply could do with some percent to power the message
home a bit, however instead of that, that
is fantastic blog. An excellent read. I will definitely be back.

Rispondi
rental market survey 11 Luglio 2015 - 0:01

I like the helpful information you provide in your articles.
I’ll bookmark your blog and check again here regularly.
I am quite certain I will learn many new stuff right here!

Good luck for the next!

Rispondi
forecast 11 Luglio 2015 - 0:05

What’s up, yup this piece of writing is actually fastidious
and I have learned lot of things from it about
blogging. thanks.

Rispondi
1 bedroom apartments for rent vancouver 11 Luglio 2015 - 2:31

Good day! Would you mind if I share your blog with my
twitter group? There’s a lot of folks that I think would really enjoy
your content. Please let me know. Cheers

Rispondi
sneak a peek here 11 Luglio 2015 - 3:23

Since the admin of this site is working, no question very shortly it will be renowned, due to its feature contents.

Rispondi
real estate vancouver 11 Luglio 2015 - 3:26

fantastic submit, very informative. I ponder why the other experts of this sector do not notice
this. You should continue your writing. I am sure, you have
a great readers’ base already!

Rispondi
real estate vancouver 11 Luglio 2015 - 3:43

I am really loving the theme/design of your blog.
Do you ever run into any browser compatibility problems? A few of
my blog visitors have complained about my site not operating
correctly in Explorer but looks great in Firefox.
Do you have any advice to help fix this problem?

Rispondi
websites 11 Luglio 2015 - 12:02

Inspiring quest there. What happened after?
Good luck!

Rispondi
condo for sale vancouver 11 Luglio 2015 - 19:51

I like the valuable info you provide in your articles.

I’ll bookmark your weblog and check again here frequently.

I am quite sure I’ll learn lots of new stuff right
here! Best of luck for the next!

Rispondi
Security Guard Agencies Georgia 11 Luglio 2015 - 21:14

Appreciate this post. Will try it out.

Rispondi
Dunwoody Security Patrol Companies 13 Luglio 2015 - 2:35

Heya i am for the first time here. I came across this board
and I find It truly useful & it helped me out much.
I hope to give something back and aid others like you helped me.

Rispondi
Security Guards Cobb County 13 Luglio 2015 - 19:46

wonderful put up, very informative. I’m wondering why the other experts of this
sector do not understand this. You must proceed your writing.
I am sure, you have a great readers’ base already!

Rispondi
Licensed Moving Companies in Newcastle Oklahoma 17 Luglio 2015 - 3:17

Hurrah! Finally I got a website from where I be able to actually get
valuable data concerning my study and knowledge.

Rispondi
รับทำ seo fanpage 24 Luglio 2015 - 9:28

Hello there, just became alert to your blog through Google,
and found that it is really informative. I am gonna watch out
for brussels. I will be grateful if you continue this in future.
Lots of people will be benefited from your writing. Cheers!

Rispondi
ยูนิฟอร์ม 24 Luglio 2015 - 23:42

wonderful issues altogether, you just received a
emblem new reader. What may you suggest in regards to your put up
that you just made a few days ago? Any positive?

Rispondi
พิมพ์กดแซนวิช 25 Luglio 2015 - 5:42

If some one wants to be updated with most recent technologies afterward he must be pay a quick visit this web site and be up to date every day.

Rispondi
เสื้อชีฟอง 25 Luglio 2015 - 10:36

If you would like to increase your know-how only keep visiting this website and be updated with the most
up-to-date information posted here.

Rispondi
รับทำ seo fanpage 25 Luglio 2015 - 22:54

I do not even know how I ended up here, but I thought this post was good.

I don’t know who you are but certainly you’re going to a famous blogger if you aren’t already 😉 Cheers!

Rispondi
steam download 26 Luglio 2015 - 19:23

This is a topic that’s near to my heart…
Cheers! Exactly where are your contact details though?

Rispondi
steam games 27 Luglio 2015 - 10:37

Good day! Do you know if they make any plugins to help with Search Engine
Optimization? I’m trying to get my blog to rank for some targeted keywords
but I’m not seeing very good gains. If you know of any please
share. Thank you!

Rispondi
รับสกรีนเสื้อ 28 Luglio 2015 - 21:13

I got this site from my buddy who informed me about this web page and at the moment
this time I am visiting this web page and reading very informative content here.

Rispondi
เสื้อผ้าแฟชั่นราคาถูก 2 Agosto 2015 - 10:53

Thanks on your marvelous posting! I really enjoyed reading it, you will
be a great author.I will always bookmark your blog and
will come back in the foreseeable future.
I want to encourage you to continue your great work, have a nice day!

Rispondi
https://agariohackonline.wordpress.com/ 15 Agosto 2015 - 2:58

You could take to staying with fishies your size, specially
in empty areas.

Rispondi

Lascia il tuo commento

Inserisci la somma corretta Limite di tempo superato. Si prega di completare nuovamente il captcha.

Il Fertilitycenter.it è un sito informativo al fianco delle coppie per sostenerle ed informarle nel desiderio più grande, quello di avere un figlio.

ULTIMI ARTICOLI

fertilitycenter.it © 2023 All right reserved.

Questo sito Web utilizza i cookie per migliorare la tua esperienza. Supponiamo che tu sia d'accordo con questo, ma puoi annullare l'iscrizione se lo desideri. Accetto Leggi

-
00:00
00:00
Update Required Flash plugin
-
00:00
00:00